Table 3.
Sixteen VH/Vκ and VH/Vλ gene couples derived from single B cells of three Wegener's granulomatosis (WG) tissue samples.
| Patient no. | Sequence | Germline gene | Homology (% to germline) | CDR | FR | CDR 3 length | ||
|---|---|---|---|---|---|---|---|---|
| R | S | R | S | |||||
| 1 | Hm0507-10-VH4 | 4-39 | 89 | 6 | 2 | 7 | 5 | 16 |
| Hm0507-10-VK2 | 2-28 | 99 | 0 | 0 | 1 | 0 | 7 | |
| 1 | Hm0207-17-VH1 | 1-69 | 99 | 0 | 0 | 1 | 1 | 12 |
| Hm0207-17-VL1 | 1-74 | 99 | 3 | 0 | 6 | 2 | 13 | |
| 1 | Hm0407-08-VH4 | 4-39 | 90 | 5 | 1 | 9 | 6 | 18 |
| Hm0407-08-VL1 | 1-44 | 94 | 3 | 2 | 8 | 4 | 11 | |
| 1 | Hm0507-12-VH1 | 1-46 | 93 | 4 | 2 | 4 | 3 | 17 |
| Hm0507-12-VL1 | 1-40 | 98 | 2 | 0 | 2 | 0 | 11 | |
| 1 | Hm1207-35-VH3 | 3-21 | 92 | 3 | 0 | 6 | 2 | 20 |
| Hm1207-35-Vκ3 | 3-11 | 98 | 0 | 0 | 2 | 0 | 9 | |
| 2 | Scu3-VH4 | 4-4 | 90 | 6 | 0 | 5 | 9 | 11 |
| Scu3-VL6 | 6-57 | 99 | 1 | 0 | 1 | 0 | 9 | |
| 2 | Scu68-VH4 | 4-61 | 85 | 8 | 4 | 12 | 7 | 17 |
| Scu68-VL3 | 3-19 | 85 | 0 | 0 | 4 | 1 | 12 | |
| 2 | Scu0107-36-VH4 | 4-31 | 96 | 1 | 1 | 7 | 0 | 13 |
| Scu0107-36-VK1 | 1-16 | 97 | 1 | 1 | 2 | 1 | 9 | |
| 2 | Scu0107-58-VH3 | 3-30 | 88 | 5 | 3 | 9 | 3 | 21 |
| Scu0107-58-VK3 | 3-15 | 91 | 3 | 1 | 11 | 4 | 10 | |
| 2 | Scu0107-42-VH1 | 1-18 | 98 | 2 | 0 | 1 | 2 | 17 |
| Scu0107-42-VL1 | 1-36 | 98 | 0 | 0 | 2 | 2 | 11 | |
| 2 | Scu0107-45-VH1 | 1-18 | 89 | 6 | 1 | 12 | 5 | 15 |
| Scu0107-45-VL2 | 2-14 | 98 | 3 | 0 | 0 | 0 | 10 | |
| 2 | Scu0107-53-VH1 | 1-3 | 99 | 1 | 0 | 0 | 0 | 17 |
| Scu0107-53-VL3b | 3-1 | 96 | 3 | 1 | 3 | 2 | 9 | |
| 2 | Scu0107-59-VH1 | 1-18 | 92 | 2 | 1 | 10 | 2 | 19 |
| Scu0107-59-VL3b | 3-1 | 96 | 4 | 0 | 1 | 3 | 9 | |
| 3 | ST140-VH4 | 4-59 | 89 | 3 | 0 | 10 | 6 | 9 |
| ST140-VK1 | 1-12 | 100 | 0 | 0 | 0 | 0 | 10 | |
| 3 | ST146-VH4 | 4-34 | 92 | 2 | 2 | 9 | 3 | 14 |
| ST146-VK2 | 2D-40 | 99 | 0 | 0 | 1 | 0 | 10 | |
| 3 | ST177-VH3 | 3-48 | 86 | 0 | 0 | 7 | 4 | 17 |
| ST177-VK5 | 5-2 | 99 | 0 | 0 | 1 | 0 | 11 | |
Germline gene and homology indicate the percentage of identical nucleic acids compared with the respective germline gene. The total number of replacement (R) and silent (S) mutations within the complementarity determining region (CDR) and the framework region (FR) are listed. The CDR3 length is given in amino acid numbers.