Fig. 2.

Percentage of phagocytosis. Peritoneal macrophages were obtained and seeded in tissue culture plates containing coverslips, and stimulated in vitro with 50 µg/ml of soluble Tityus serrulatus (TSV), partially purified fraction (FII) or pure toxin (Ts1) or 5 µg/ml of lipopolysaccharide (LPS), as described in Material and methods. Three coverslips per experimental condition were used to determine the percentage of phagocytic cells. Each point represents the mean ± standard deviation value of samples from four experiments carried out on different groups each consisting of five mice. Statistical differences between the expositions were P < 0·001.