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Typical effect of Tityus serrulatus (TSV) or toxins on macrophages. Time lapse transmitted light images (Normarsky interference) exposed to TSV scorpion venom. (a) Time 0; (b) 30 min; (c) 45 min after addition of purified pure toxin (Ts1). Intracellular Ca2+ concentration (FLUO3 fluorescence) of the same cells at the same time-points (d, e, f). Note the increment and decrement of the Ca2+ signal and the changes in macrophage morphology.
