Table 1.
Essential E. coli genes with high ROL values are also essential in Acinetobacter.
| K12 gene | Gene function | ROL γ-β | ROL α | ROL B-M | Essential |
| asd | ASA dehydrogenase | 3.07 | n.a. | n.a. | DB |
| can | carbonic anhydrase | 4.07 | 11.67 | n.a. | - |
| degS | serine endoprotease | 8.63 | 9.54 | 7.77 | NON |
| dnaC | DNA biosynthesis protein | 62.30 | n.a. | 94.06 | - |
| fabA | HD thioester dehydrase | 2.78 | 2.42 | n.a. | - |
| fabB | 3-oxoacyl-[acp] synthase | 3.55 | 2.75 | n.a. | DB |
| fbaA | FBP aldolase | 3.21 | n.a. | n.a. | - |
| fldA | flavodoxin 1 | 4.73 | 22.92 | 16.86 | - |
| ftsE | transporter subunit | 3.05 | 2.48 | 8.79 | - |
| ftsK | chromosome partitioning | 7.20 | 2.55 | 10.41 | ESS |
| ftsL | cell division protein | 4.59 | n.a. | n.a. | - |
| ftsN | cell division protein | 12.64 | n.a. | n.a. | - |
| ftsX | transporter subunit | 2.87 | 13.32 | 9.10 | - |
| hemD | uroporphyrinogen synthase | 2.75 | n.a. | n.a. | DB |
| hemG | protoporphyrin oxidase | 8.47 | n.a. | n.a. | - |
| holB | DNA ROL III subunit | 5.08 | 19.02 | 32.24 | - |
| lolB | chaperone for lipoproteins | 2.67 | n.a. | n.a. | ESS |
| lolE | lipoprotein transporter | 7.92 | 5.89 | 55.12 | - |
| mreD | cell wall structural complex | 3.53 | n.a. | n.a. | - |
| mukB | chromosome partitioning | 13.42 | n.a. | 94.78 | - |
| mukE | chromosome partitioning | 7.41 | n.a. | n.a. | - |
| mukF | chromosome partitioning | 7.16 | n.a. | n.a. | - |
| nrdA | RDP reductase subunit | 4.91 | n.a. | n.a. | - |
| nrdB | RDP reductase subunit | 3.03 | 5.75 | n.a. | ESS |
| plsB | O-acyltransferase | 5.09 | n.a. | n.a. | ESS |
| plsC | acyltransferase | 4.06 | 5.79 | 32.81 | - |
| psd | decarboxylase | 3.28 | 36.74 | 13.10 | ESS |
| pssA | phosphatidylserine synthase | 7.12 | n.a. | n.a. | - |
| rlpB | minor lipoprotein | 8.13 | n.a. | n.a. | - |
| secM | regulator of secA | 10.80 | n.a. | n.a. | - |
| yejM | hydrolase, inner membrane | 6.76 | n.a. | n.a. | - |
| yrbK | conserved protein | 8.94 | n.a. | n.a. | - |
| yrfF | inner membrane protein | 7.93 | n.a. | n.a. | - |
| zipA | cell division protein | 3.33 | n.a. | n.a. | - |
Only those E. coli K12 genes with high ROL values in γ-β-proteobacteria, α-proteobacteria, and Bacilli-Mollicutes (ROL values greater than 2.4 in all three groups (Fig. 1)) are shown here. Of these 34 genes, nine have an orthologue in Acinetobacter, and eight of these have an essential function in Acinetobacter. Many of the genes lost at high rates from γ-β-proteobacteria have orthologues in fewer than 10% of the α-proteobacteria or Bacilli-Mollicutes; these are indicated by n.a. ESS, genes for which no deletion mutants were isolated in Acinetobacter; DB, genes in which only "double band" mutants containing chromosomal duplications were isolated in Acinetobacter (and which are thus likely to be essential); NON, genes for which deletion mutants were isolated in Acinetobacter, and which are thus considered to be non-essential; [9]. '-', genes without an orthologue in Acinetobacter.