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. 2009 Feb 1;20(3):904–914. doi: 10.1091/mbc.E08-08-0835

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© 2009 by The American Society for Cell Biology

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Figure 5. — Domain localization of bimodal Centrin and Sas6a basal body incorporation. (A) Centrin IEM localization in starved (no basal body duplication) or asynchronous (incorporation at duplicated basal bodies) cells expressing GFP-Cen1. The majority of Cen1 loaded at the basal body distal end, independent of new basal body assembly. GFP-Cen1 incorporated at all three major Cen1-localizing domains after stable and dynamics incorporation during new basal body assembly. (B) Both dynamic (starved) and dynamic and stable (asynchronous) populations of GFP-Sas6a predominantly localize to the cartwheel hub and spokes. On new basal body assembly, the GFP-Sas6a is more focused at the cartwheel. Each condition represents a total count of at least 150 gold particles for at least 30 basal bodies. Cartoons display the relative fraction of gold localization to each domain with a total of 25 red spots. Bar, 100 nm.