Table 2.

Relative fluorescence of new and old basal bodies adjacent to each other in live cells after 2 h of GFP-fusion protein expression

	Fluorescence ratio (old/new)a
GFP-Cen1	0.32 ± 0.13 (230)
GFP-Sas6a	0.38 ± 0.18 (211)
GFP-Atu1	0.11 ± 0.20 (323)
GFP-Spag6b	0.98 ± 0.32 (213)

^a The fluorescence ratio is the corrected fluorescence for the old (dim) basal body divided by the new (bright) basal body fluorescence. This ratio defines the fraction of basal body protein that is assembled independent of nascent basal body assembly. Basal body pairs in close but resolvable proximity were quantified if they were present in either quadrant II or quadrant III in cells with a developing oral apparatus. Parentheses describe the number (n) of basal body pairs for each experiment.

^b It was not possible to discern new and old basal bodies with GFP-Spag6 due to dynamic protein exchange throughout the cell cycle causing equal fluorescence intensities.