Figure 3.

Expression of K-Ras on apoptosis are reversed by COX-2 inhibition. (A) The indicated cells were grown for 3 d in 2D culture or 5 d in 3D Matrigel culture in the presence or absence of NS-398 as shown in Figure 2. Cells were fixed and then exposed to TUNEL stain. Representative fields are shown with TUNEL-positive cells stained in green. The majority of fields of K-Ras cells showed no TUNEL-positive cells. (B) Extracts were prepared from each of the cell populations and immunoblotted for cleaved caspase-3. Results are representative of three independent experiments.