Figure 4.

K-Ras lowers adherens junctions and transepithelial resistance. (A) Cells were plated on eight-well chamber slides and grown for 48 h to achieve a confluent monolayer, and then they were treated with 5 μM ionomycin or vehicle (control) for 30 min at 37°C. Cells were then fixed and stained with anti-E-cadherin antibody to view cell adherens junctions. Representative pictures shown are from three independent experiments. (B) Cells were plated at a density of 50,000 cells/transwell in growth medium overnight. Then cells were switched to BPE-free medium and TER was measured daily, as described in Materials and Methods. Data represent the mean from four independent experiments. *p < 0.05 versus K-Ras.