Figure 5.

K-Ras promotes E-cadherin shedding. (A) Cell lysates from vector control and K-Ras cells were immunoblotted with the indicated antibodies. (B) Sequence analysis of MMP-9 LSD cutting sites in both human and rat E-cadherin. (C) Cell lysates prepared from vector control (V) or K-Ras–transfected (K) cells were immunoprecipitated with two anti-E-cadherin antibodies, 4A2C7 and H108, and the immunoprecipitates were immunoblotted with another specific antibody that recognizes the carboxy terminal of the molecule (C36). Arrows indicate predicted specific cleavage products at 42 and 29 kDa. The 120-kDa band represents full-length E-cadherin. HC, immunoglobulin G heavy chain.