Figure 8.

TOPFLASH and E-cadherin cleavage in human NSCLC expressing oncogenic K-Ras. (A) H2122 (left) and A549 cells (right) were transiently transfected with TOPFLASH (TF) or FOPFLASH (FF) promoter as a negative control. After transfection, cells were with NS-398 or vehicle (control) for 24 h, and promoter activity was normalized to β-gal determined. NS-398 decreased TOPFLASH in both cell lines. (B) E-cadherin shedding occurs in both cell lines. The 42-kDa E-cadherin fragment was detected in anti-E-cadherin immunoprecipitations followed by immunoblotting with a different E-cadherin antibody.