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. 2009 Feb 1;20(3):870–881. doi: 10.1091/mbc.E08-07-0671

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© 2009 by The American Society for Cell Biology

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Figure 3. — Validation of the interaction between TP53INP2 and its partners. (a) Coimmunoprecipitation assays. 293T cells were transfected with the TP53INP2-EGFP construct alone or in combination with FLAG-GABARAP, FLAG-GABARAP-like2, or FLAG-LC3 plasmids. Lysates were immunoprecipitated with a FLAG antibody and revealed by a GFP antibody. (b) Yeast two-hybrid cotransformation. pSos-TP53INP2, pSos-TP53INP2-N-term, or pSos-TP53INP2-C-term were combined with pMyr-GABARAP, pMyr-gGbarap-like2, or pMyr-LC3 to transform yeasts. Yeast cells were plated on Gal-U/L for growth selection of bait–prey interaction. (c) Interaction of TP53INP2 with VMP1. Coimmunoprecipitation assay: 293T cells were transfected with the TP53INP2-V5 construct alone or in combination with the VMP1-EGFP construct. Lysates were immunoprecipitated with a GFP antibody and revealed with a V5 antibody. IP, immunoprecipitation; TCL, total cell lysate.