Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Feb 1;20(3):1030–1047. doi: 10.1091/mbc.E08-06-0637

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Cell Biology

PMC Copyright notice

Figure 6. — rec8-29A mutant cells exhibit defects in homologous recombination. (A) Wild-type (A1556), rec8Δ (A18933), and pREC8-SCC1 rec8Δ (A16132) were induced to sporulate. At the indicated times, cells were harvested and assayed by Southern blot for DSBs and recombination products at HIS4/LEU2. (B) Wild-type (A1556), rec8Δ (A18933), rec8-29A (A21618), and rec8-6A (A18936) cells were induced to sporulate. At the indicated times, cells were harvested and assayed by Southern blot for DSBs and recombination products at HIS4/LEU2. (C and D) Blots from A and B were subjected to densitometric analysis to quantitate the intensity of the bands representing the upper recombinant band. Values were normalized to the “Mom” parental band for each strain and each time point. (E) Meiotic progression of the strains assayed for recombination shown in A and B. Wild-type (A1556, ◇), rec8Δ (A18933, ■), pREC8-SCC1 rec8Δ (A16132, ○), rec8-29A (A21618, ·), and rec8-6A (A18936, ▴) cells were induced to sporulate. At the indicated times, samples were taken and subjected to α-tubulin immunofluorescence to determine the percentage of cells with unassembled spindles. n = 200 cells counted per strain per time point.