Table 1.
Yeast strains used in this study
| Strain number | Relevant genotype |
|---|---|
| A1556 | MATa/α his4B::LEU2 his4X::LEU2-(BamH1) |
| A1972 | MATa/α REC8-3HA::URA3 |
| A3528 | MATa/α rec8Δ::KanMX4 |
| A5111 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 |
| A5844 | MATa/α cdc5::pCLB2-CDC5::KanMX6 |
| A6946 | MATa/α pURA3-tetR-GFP::LEU2 ura3::tetOx224::URA3 ndt80::URA3 |
| A6917 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 dmc1::KanMX |
| A7097 | MATa/α |
| A7803 | MATa/α pURA3-tetR-GFP::LEU2 ura3::tetOx224::URA3 spo11::TRP1 ndt80::URA3 |
| A8477 | MATa/α spo11::URA3 |
| A9115 | MATa/α pURA3-tetR-GFP::LEU2 ura3::tetOx224::URA3 spo11::spo11-Y135F-HA::URA3 ndt80::LEU2 |
| A9828 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 |
| A9829 | MATa/α lys2::tetOx240::URA3 (heterozygous) leu2::LEU2-tetR-GFP pURA3-tetR-GFP::LEU2 ura3::tetOx224::URA3 (heterozygous) ndt80::URA3 |
| A10404 | MATa/α pURA3-tetR-GFP::LEU2 ura3::tetOx224::URA3 ndt80::URA3 cdc6::KanMX6::pSCC1-CDC6-3HA |
| A10735 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 cdc6::KanMX6::pSCC1-CDC6-3HA |
| A11469 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 spo11::URA3 |
| A11268 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 clb6::TRP1 clb5::KanMX6 |
| A11326 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 clb6::TRP1 clb5::KanMX6 |
| A11474 | MATa/α lys2::tetOx240::URA3 (heterozygous) leu2::LEU2-tetR-GFP leu2::LEU2-tetR-GFP-tetO-HIS3 (heterozygous) ndt80::URA3 |
| A12095 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 spo11::spo11-Y135F-HA::URA3 |
| A13346 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 spo11-Y135F-HA::KanMX4 |
| A13539 | MATa/α pREC8::REC8-3HA-NC rec8::KanMX4 |
| A13946 | MATa/α pREC8::REC8-3HA::KanMX4::LEU2 |
| A14385 | MATa/α pREC8::rec8-29A-3HA::LEU2 rec8::KanMX4 |
| A14655 | MATa/α pREC8::REC8-3HA::KanMX4::LEU2 ubr1::KanMX4 |
| A15042 | MATa/α pREC8::rec8-6A-3HA::LEU2 rec8::KanMX4 |
| A15364 | MATa/α pREC8::rec8-psa-3HA::LEU2 rec8::KanMX4 ubr1::KanMX4 |
| A15880 | MATa/α cdc6:: pSCC1-3HA-CDC6::KanMX6 |
| A16108 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 rec8::LEU2 |
| A16113 | MATa/α clb5::KanMX6 clb6::TRP1 pREC8::REC8-3HA::URA3 |
| A16126 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 spo11-D290A-HA::KanMX4 |
| A16131 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 rec8::LEU2 |
| A16132 | MATa/α pREC8::pREC8-SCC1-3HA::LEU2 rec8::KanMX4 his4B::LEU2 his4X::LEU2-(BamH1) |
| A16133 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 spo11-Y135F-HA::KanMX4 |
| A16147 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 SPO11-HA::KanMX4 (heterozygous) spo11-Y135F::KanMX4 (heterozygous) |
| A16148 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 SPO11-HA::KanMX4 (heterozygous) spo11-Y135F-HA::KanMX4 (heterozygous) |
| A16149 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 mer2::mer2-S30A::URA3 |
| A16290 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 msh5::HIS3 |
| A16292 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 SPO11-HA::KanMX4 |
| A16360 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 (heterozygous) CENV::tetOx224::HIS3 (heterozygous) leu2::pURA3-tetR-GFP ndt80::URA3 |
| A16362 | MATa/α CENV::tetOx224::HIS3 leu2::pURA3-tetR-GFP ndt80::URA3 |
| A16366 | MATa/α pURA3-tetR-GFP::LEU2 TELV::tetOx224::URA3 ndt80::URA3 |
| A16376 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 SPO11-HA::KanMX4 |
| A16386 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 SPO11-HA::KanMX4 (heterozygous) spo11-Y135F-HA::KanMX4 (heterozygous) |
| A16391 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 SPO11-HA::KanMX4 (heterozygous) spo11-Y135F::KanMX4 (heterozygous) |
| A16399 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 spo11-D290A-HA::KanMX4 |
| A16412 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 pREC8::rec8-29A-3HA::LEU2 rec8::KanMX4 |
| A16446 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 cdc6::KanMX6::pSCC1-CDC6-3HA rec8::LEU2 |
| A16460 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 cdc6::KanMX6::pSCC1-CDC6-3HA rec8::LEU2 |
| A16533 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 cdc6::KanMX6::pSCC1-CDC6-3HA |
| A16535 | MATa/α pURA3-tetR-GFP::LEU2 TELV::tetOx224::URA3 ndt80::URA3 rec8::LEU2 |
| A16537 | MATa/α CENV::tetOx224::HIS3 leu2::pURA3-tetR-GFP ndt80::URA3 rec8::LEU2 |
| A16538 | MATa/α pURA3-tetR-GFP::LEU2 ura3::tetOx224::URA3 ndt80::URA3 rec8::LEU2 |
| A16664 | MATa/α rec8::KanMX4 |
| A17021 | MATa/α cdc6:: pSCC1-3HA-CDC6::KanMX6 rec8::KanMX4 |
| A18933 | MATa/α his4B::LEU2 his4X::LEU2-(BamH1) rec8::KanMX4 |
| A18936 | MATa/α his4B::LEU2 his4X::LEU2-(BamH1) pREC8::rec8-6A-3HA::LEU2 rec8::KanMX4 |
| A19798 | MATa/α spo11Δ::URA3 ndt80Δ::URA3 ura3::pGPD1-GAL4(848)-ER::URA3 rec8::pGAL-REC8-UP-3HA::KanMX6 lys2::tetOx240::URA3 leu2::tetR-GFP::LEU2 |
| A19800 | MATa/α spo11Δ::URA3 ndt80Δ::URA3 ura3::pGPD1-GAL4(848)-ER::URA3 rec8::pGAL-REC8-3HA::KanMX6 lys2::tetOx240::URA3 leu2::tetR-GFP::LEU2 |
| A20066 | MATa/α pREC8::REC8-3HA::KanMX4::LEU2 |
| A20072 | MATa/α pREC8::REC8-T173D S197D S245D S386D S387D S521D-3HA::KanMX4::LEU2 |
| A20075 | MATa/α MATa/α pREC8::REC8-T173E S197E S245E S386E S387E S521E-3HA::KanMX4::LEU2 |
| A20151 | MATa/α pREC8::rec8-6A-3HA::LEU2 rec8::KanMX4 pch2Δ::KanMX4 |
| A20153 | MATa/α pREC8::REC8-3HA::KanMX4::LEU2 pch2::KanMX4 |
| A20154 | MATa/α pREC8::rec8-6A-3HA::LEU2 rec8Δ::KanMX4 mek1::KanMX |
| A20156 | MATa/α pREC8::REC8-3HA::KanMX4::LEU2 mek1::KanMX |
| A20157 | MATa/α pREC8::rec8-29A-3HA::LEU2 rec8::KanMX4 mek1::KanMX |
| A20163 | MATa/α pSCC3::pCLB2-SCC3 scc3::KanMX6 |
| A20164 | MATa/α pREC8::rec8-29A-3HA::LEU2 rec8::KanMX4 pch2::KanMX4 |
| A21230 | MATa/α ura3::pGPD1-GAL4(848)-ER::URA3 rec8::KanMX4::REC8-3HA::LEU2 pGAL-NDT80::TRP1 |
| A21232 | MATa/α ura3::pGPD1-GAL4(848)-ER::URA3 rec8::KanMX4::rec8-29A-3HA::LEU2 pGAL-NDT80::TRP1 |
| A21234 | MATa/α ura3::pGPD1-GAL4(848)-ER::URA3 rec8::KanMX4::rec8-17A-3HA::LEU2 pGAL-NDT80::TRP1 |
| A21463 | MATa/α spo11::TRP1 pREC8::rec8-6A-3HA::LEU2 rec8::KanMX4 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21464 | MATa/α spo11::TRP1 pREC8::rec8-6A spo13::KanMX -3HA::LEU2 rec8::KanMX4 leu2::tetR-GFP-tetO::LEU2::HIS3 |
| A21465 | MATa/α spo11::TRP1 pREC8::rec8-6A-3HA::LEU2 rec8::KanMX4 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21466 | MATa/α spo11::TRP1 pREC8::REC8-3HA::LEU2 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21467 | MATa/α spo11::TRP1 pREC8::REC8-3HA::LEU2 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21468 | MATa/α spo11::TRP1 pREC8::REC8-3HA::LEU2 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21469 | MATa/α spo11::TRP1 pREC8::rec8-29A-3HA::LEU2 rec8::KanMX4 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21470 | MATa/α spo11::TRP1 pREC8::rec8-29A-3HA::LEU2 rec8::KanMX4 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21471 | MATa/α spo11::TRP1 pREC8::rec8-29A-3HA::LEU2 rec8::KanMX4 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21472 | MATa/α spo11::TRP1 rec8::KanMX4 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21473 | MATa/α spo11::TRP1 rec8::KanMX4 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21474 | MATa/α spo11::TRP1 rec8::KanMX4 leu2::tetR-GFP-tetO::LEU2::HIS3 spo13::KanMX |
| A21618 | MATa/α his4B::LEU2 his4X::LEU2-(BamH1) pREC8::rec8-29A-3HA::LEU2 rec8::KanMX4 |
| A21668 | MATa/α lys2::tetOx240::URA3 leu2::LEU2-tetR-GFP ndt80::URA3 pREC8::rec8-6A-3HA::LEU2 rec8::KanMX4 |
| A21669 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 pREC8::rec8-29A-3HA::LEU2 rec8::KanMX4 |
| A21670 | MATa/α leu2::LEU2::tetR-GFP-tetO::HIS3 ndt80::URA3 pREC8::rec8-6A-3HA::LEU2 rec8::KanMX4 |