Figure 3.

Three distinct classes of misfolded proteins induce unique patterns of transcription. Cells expressing galactose-inducible constructs were harvesting for RNA processing and Northern blotting at 0, 0.5, 1, 3, 6, and 12 h after galactose induction. Shown is a representative experiment using the wild-type strain (SM4460) expressing empty vector (pSM922), ste6-Q1249X (pSM2213), CPY* (pSM2215), and VHL-L158P (pESC-L158P). (A) The indicated probes detect the UPR-L genes, KAR2, YFR026C, and HAC1, splicing. (B) Probes detect the cytosolic chaperones, SSA4 and STI1. Northern blots were performed in duplicate and shown is a representative experiment in which quantitated, normalized values, determined as described in Materials and Methods, are graphed as percent of HAC1 spliced or arbitrary units.