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. 2009 Feb 1;20(3):1006–1019. doi: 10.1091/mbc.E08-02-0140

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© 2009 by The American Society for Cell Biology

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Figure 4. — The UPR-M/C response is characterized by the induction of Rpn4p-target genes. (A) Wild-type cells (SM4460) expressing galactose-inducible ste6-Q1249X (pSM2213) were harvested for RNA processing at 0, 0.5, 1, 3, 6, and 12 h after galactose induction, and Northern blotting was performed with probes to the indicated UPR-M/C genes. (B) Northern blot analysis of wild-type cells (SM4460) expressing galactose-inducible empty vector (pSM922), ste6-G38D (pSM1898), ste6-L1239X (pSM2212), ste6-Q1249X (pSM2213), CPY* (pSM2215), or VHL-L158P (pESC-L158P) were harvested for RNA processing and Northern blotting with RPN3 at the indicated times after galactose induction. Northern blots were performed in duplicate and shown is a representative experiment in which quantitated, normalized values, determined as described in Materials and Methods, are graphed as arbitrary units.