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. 2009 Feb 1;20(3):1006–1019. doi: 10.1091/mbc.E08-02-0140

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© 2009 by The American Society for Cell Biology

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Figure 6. — Rpn4p is required to survive UPR-M/C stress, but not UPR-L or UPR-Cyto stress. (A) Serial dilutions of the rpn4Δ mutant (SM5383) containing wild-type STE6 (pSM1897), empty vector (pSM922), or the UPR-M/C stresses ste6-G38D (pSM1898), ste6-L1239X (pSM2212), or ste6-Q1249X (pSM2213) are shown. (B) Serial dilutions of the rpn4Δ mutant (SM5383) containing plasmids that induce the UPR-Cyto stress, VHL (pESC-VHL) or VHL-L158P (pESC-L158P), or the UPR-L stress, CPY* (pSM2215) are shown. (C) Serial dilutions of wild-type (SM4460), rpn4Δ (SM5383), hac1Δ (SM5382), and ssh1Δ (SM5384) cells were grown on media containing 0.1 μg/ml tunicamycin or an equal volume DMSO for 2 d at 30°C.