Table 2.
Plasmids used in this study
| Plasmid | Genotype | Reference or source |
|---|---|---|
| pESC-VHL | [2μ URA3 PGAL1 VHL] | McClellan et al. (2005) |
| pESC-L158P | [2μ URA3 PGAL1 VHL-L158P] | McClellan et al. (2005) |
| pPW344 | [2μ URA3 4 × UPRE-LacZ] | Patil et al. (2004) |
| pSM922 | [2μ URA3 PGAL1] | Mumberg et al. (1994) |
| pSM1897 | [2μ URA3 PGAL1 STE6::GFPc] | This study |
| pSM1898 | [2μ URA3 PGAL1ste6-G38D::GFPc] | This study |
| PSM2212 | [2μ URA3 PGAL1ste6-L1239X] | This study |
| pSM2213 | [2μ URA3 PGAL1ste6-Q1249X] | This study |
| pSM2215 | [CEN URA3 PGAL1 CPY*] | This study |
| pSM2216 | [CEN LEU2 PPGK Ub-Pro-LacZ] | This study |
| pSM2217 | [2μ URA3 PGAL1pma1-D378S] | This study |