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. 2009 Feb 1;20(3):882–890. doi: 10.1091/mbc.E08-07-0731

Figure 6.

Figure 6.

Significantly decreased colony-forming efficiency of Smad4 mutant keratinocytes. (A and B) Flow cytometry of α6-integrin and CD34 double-labeled keratinocytes from dorsal epidermis of wild-type and Smad4 mutant mice at P72. (C–J) Clonal forming assay with 1 × 105 total keratinocytes from 6- to 8-wk-old control (C, E, G, and I) and Smad4 mutant mice (D, F, H, and J). Microscopic view of wild-type (E) and Smad4 mutant (F) colonies. Immunofluorescence on wild-type and Smad4 mutant keratinocytes in culture for K14 (G and H), involucrin (I and J), and Ki67 (K–R). Bar, (E–J) 20 μm; (K and L) 25 μm; (M–R) 50 μm.