Figure 10.

HU induces increased levels of phosphorylation of histone H2Av in stwl⁸⁴ mutants. (A) An antiserum directed against H2Av phosphorylated at Ser137 (γ-H2Av) on histone isolations was used as a marker for DNA damage induced by HU in wild-type and stwl⁸⁴ homozygous larvae after 50 mg/ml HU treatment for 24 h. HU induces accumulation of γ-H2Av in wild-types and in stwl⁸⁴ mutants; however, in stwl⁸⁴ mutants, a larger increase of γ-H2Av levels was detected compared with wild-types. H2A was used as a loading control. (B) The average ratio of γ-H2Av expression levels to H2A expression levels was quantified using four independent experiments.