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. Author manuscript; available in PMC: 2009 Jan 30.
Published in final edited form as: Angew Chem Int Ed Engl. 2008;47(35):6661–6665. doi: 10.1002/anie.200802199

Figure 1.

Figure 1

Ribosome inactivating protein mediated depurination of the α-sarcin/ricin hairpin RNA substrate 1 at position A15 (corresponding to A4324 of rat 28S rRNA) yields an RNA product 2 a containing an abasic site. Highlighted residues 9–20 represent the longest conserved RNA sequence in the large ribosomal subunit.[6] RNA 2 b represents a model depurinated RNA product that contains a stable THF residue as an abasic site mimic. Also shown are the chemically synthesized fluorescent probes 3–6, which contain the emissive nucleobase analogue 7, used in this study.