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. 2008 Feb 13;28(7):1697–1708. doi: 10.1523/JNEUROSCI.3032-07.2008

Figure 2.

Figure 2.

Altered composition of PSD fractions in Shank1 mutant mice. Analysis of Triton-extracted PSD fractions (PSDI) purified from forebrain of adult wild-type (+/+) and Shank1 knock-out (−/−) mice. A, SDS-PAGE and silver staining of PSD proteins revealed no major differences. B, Immunoblot analysis of PSD fractions from individual wild-type and Shank1−/− mice for the indicated proteins. C, Quantitation of various proteins in PSD fractions based on results as shown in B, normalized to wild-type (100%; black histograms). Histograms show mean ± SEM (>6 mice from each genotype). There is a significant difference in total Shank (+/+, n = 6; −/−, n = 8; ***p < 0.0001), GKAP (+/+, −/−, n = 12 each; *p < 0.05), and Homer (+/+, n = 12; −/−, n = 14; **p < 0.002).