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. 2008 Feb 13;28(7):1697–1708. doi: 10.1523/JNEUROSCI.3032-07.2008

Figure 3.

Figure 3.

Altered immunostaining of PSD proteins in Shank1-deficient neurons. A, B, Hippocampal neurons in dissociated culture (18–19 DIV) from wild-type (+/+) or Shank1−/− mice were immunostained for the indicated proteins. A, Representative dendrites double labeled for PSD-95 (green) and with Shank1-specific antibody (1356) or pan-Shank antibody (3856) (red). Merged image is shown in color on the right. Scale bar, 5 μm. B, Dendrites immunostained for GKAP, PSD-95, Homer, or presynaptic marker Bassoon. C, Quantification of puncta density per 10 μm dendrite length for the indicated proteins (mean ± SEM). Shank1−/− dendrites show a significant reduction in pan-Shank (n = 10 cells each; ***p < 0.001) and GKAP puncta density (+/+, n = 21 cells; −/−, n = 16 cells; **p < 0.01).