FIGURE 4. Effects of doxorubicin on uMUC-1 availability in BT-20 cells - in vitro tests.
A, qRT-PCR demonstrates a significant downregulation of uMUC-1 induced by doxorubicin (p = 0.028). This effect is MUC-1 specific because 18s RNA is not downregulated (inset). B, Western blot demonstrates a noticeable downregulation of uMUC-1 but not of tubulin. Arrow points to the MUC-1 band. C, TUNEL assay indicates that doxorubicin treatment of BT-20 cells induced apoptosis efficiently.