Table 5.
Gene expression changes (≥ 3-fold) examined by NCI microarray and RT-PCR in NHMEC strain 40 cells exposed for 48 hr to 10 μM TAM, compared to unexposed NHMEC strain 40 cells
| Gene | Microarray Mean Log2 ± SD | Microarray Mean fold change | Number of Micro-Arrays | RT-PCR Mean fold changeb,c |
|---|---|---|---|---|
| cig5 | 3.11 ± 0.82 | 8.7 | 10 | NA |
| IFITM1 | 3.09 ± 0.49 | 8.5 | 10 | 5.6 ± 2.9 c |
| SPP1 | 2.90 ± 0.41 | 7.5 | 10 | NA |
| KCNJ1 | 2.49 ± 0.87 | 5.6 | 12 | 3.5 ± 1.5 c |
| IFIT1 | 2.42 ± 0.65 | 5.3 | 10 | 4.4 ± 1.3 c |
| MX1 | 2.07 ± 0.65 | 4.2 | 12 | 0.5 ± 0.1 |
| GIP3 | 2.03 ± 0.73 | 4.1 | 12 | 0.6 ± 0.1 |
| IFNA1 a | NA | 5.2 ± 1.2 c |
a
NA = no assay. IFNA1 was not printed on the original microarray.
b
Each cDNA was assayed 6 times by RT-PCR, except IFNA1, which was assayed 3 times.
c
p<0.05