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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Cancer Res. 2009 Jan 20;69(3):1080–1088. doi: 10.1158/0008-5472.CAN-08-2520

Figure 5. Silencing FLIP expression enhances FasL and TRAIL-induced apoptosis in human sarcoma cells.

Figure 5

A. Comparison of cell death detection methods. HT1080 tumor cells were incubated in the absence (left 2 panels) or presence (right 2 panels) of FasL overnight and either directly stained with PI (top 2 panels), or fixed with 70% ethanol and then stained with PI as described in materials and methods (bottom 2 panel). The stained cells were analyzed by flow cytometry. B. Silencing FLIP expression increased the sensitivity of the tumor cells to FasL- and TRAIL-induced apoptosis. HT1080 cells were transiently transfected with scramble siRNA or FLIP-specific siRNA for approximately 24 h. The cells were analyzed for FLIP mRNA level using RT-PCR (left panel). The scramble- and FLIP siRNA-transfected cells were also treated with recombinant FasL or TRAIL protein overnight and analyzed for cell death by PI staining and flow cytometry analysis (right panel). Column, mean; bar, SD.