Skip to main content
. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Cancer Res. 2009 Jan 20;69(3):794–801. doi: 10.1158/0008-5472.CAN-08-2110

Figure 3. ARF6 is not required for HGF-stimulated protein phosphorylation of ERK1/2 in glioma cells.

Figure 3

Human glioma LN229, SNB19 and U373MG cell lines were transfected with siRNA for ARF6 and control then treated with 20 ng/ml of HGF for 20 min. Inhibition of ARF6 proteins in transfected cells and the impacts on ERK1/2 protein phosphorylation and GTP loading of Rac1 were analyzed with IB using anti-phospho-ERK1/2, anti-ERK1/2 and anti-ARF6 antibodies, and a Rac1 activation assay kit. IB for β-actin and Rac1 were used as protein loading controls. Data are representative of three independent experiments with similar results.