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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Nat Methods. 2009 Jan 4;6(2):161–166. doi: 10.1038/nmeth.1288

Figure 2. The QV5 construct reports the ATP/ADP ratio.

Figure 2

Application of ATP in the constant presence of three different concentrations of ADP demonstrates the increasing effective affinities of the sensor for ATP with increasing concentrations of ADP. The QV5 construct fluorescence response to ATP application in the presence of 5 μM, 50 μM, and 500 μM shows a half-maximal response at 1 μM, 10 μM, and 100 μM ATP respectively, corresponding to a half-maximal response when the ratio R equals ~0.2.

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