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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Nat Methods. 2009 Jan 4;6(2):161–166. doi: 10.1038/nmeth.1288

Figure 6. Transient glucose removal leads to a reversible change in the ATP/ADP signal.

Figure 6

(a) A cultured HEK293 cell expressing Perceval and loaded with the pH sensitive dye SNARF-5F displays an alkalinization when glucose is removed from the extracellular solution (10 mM glucose was replaced by equimolar sucrose). The Perceval signal shows a slight decrease upon glucose wash-out and a prominent rebound upon glucose wash-in. (b) The pH-corrected Perceval signal reveals a gradual decrease in cellular energy that is reversed rapidly upon glucose reapplication. Perceval occupancy of 0 corresponds to ADP ≫ ATP; occupancy of 1 corresponds to ATP ≫ ADP.

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