Figure 3.

VL12.3 increases the level of nuclear HDx-1. ST14A cells were cotransfected with mHDx-1-GFP with 103Q and intrabody at optimal ratios. A, At 48 h after transfection, cells were fixed, stained for the appropriate intrabody and cell nuclei, and analyzed by confocal microscopy. B, Mean whole-cell fluorescence intensity (int.) and mean nuclear fluorescence intensity of HDx-1 were compared. Whereas MW7, Happ1, and Happ3 have no effect on HDx-1 localization, V_L12.3 significantly increases nuclear HDx-1. *Different from HDx-1 at p < 0.01.