Figure 2.

Retinal pigment epithelium thickness and nuclei per unit length in retinal sections from wild-type and p27^Kip1 hemizygous and nullizygous mice. Measurements of retinal pigment epithelium (RPE) thickness (A) and nuclear density (B) were performed on JB-4 sections from retinas of 5 week-old p27^+/+, p27^+/- and p27^-/- mice. The apical to basal dimension of the p27^Kip1-null epithelium is, on average, approximately 6 and 12% greater than that of the hemizygous and wild-type tissues, respectively. There is also a nearly 2 fold increase in the density of nuclei for "knock-out" animals, relative to the other two genotypes (Graph of means±SEM for 5 replicates in each condition). Asterisk (*) indicates p<0.02 and double asterisks (**) indicates p<0.001 compared to wild-type.