Fig. 3.

Although appearance and disappearance of γ-H2AX is not affected by the absence of both Asf1 and Cac1, the nucleosome assembly function of Asf1 is required for the viability of asf1Δ cac1Δ cells after DSB repair. (A) γ-H2AX ChIP values at 10 kb (black bars), 20 kb (gray bars) or 30 kb (whites bars) to the right of the DSB at MAT in wild-type and asf1Δ cac1Δ cells were measured several time points (1, 2, 3, 5, 7, 9, and 12 hrs) after HO induction (see Fig. S2) and the percent maximum value of γ-H2AX ChIP is shown. (C) Percent viabilities of wild-type (black bars) and asf1Δcac1Δ (gray bars) cells with an empty plasmid, a plasmid with ASF1, or a plasmid with asf1-V94D, L96D. Cells grown in SC-Ura/Raffinose (synthetic complete medium containing raffinose instead of dextrose but lacking uracil) were plated either on YEP/Dextrose or on YEP/Galactose plates. Viability was measured as described in Fig. 1.