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Deletion of PKCλ/ι impairs CD4+ T-cell proliferation. Purified naïve CD4+ T cells from spleens or whole lymph node (LN) cells were stimulated with plate-bound anti-CD3 plus anti-CD28 for 3 days. Then cell proliferation was assessed by BrdU intracellular staining by FACS. Percentages of BrdU-positive cells are shown.
