Fig. 2.

Endogenous CHC levels are severely reduced in the chc-1(b1025ts) mutant. Gonads were dissected and immunostained with anti-CHC-1 (A–D) and anti-α-adaptin (E–H) antibodies. (A, E) wild type, 15°C; (B, F) wild type, 25°C; (C, G) chc-1(b1025ts), 15°C; (D, H) chc-1(b1025ts), 25°C. (A–H) Middle planes (Left) and enlarged images (× 4) of boxed regions in the middle planes (Right) are shown. (Scale bar, 10 μm.) (A′–D′) Fluorescence intensity of CHC staining as a function of position was graphed along a line through the center of the oocytes. (I) CHC protein level is dramatically reduced in chc-1(b1025ts) mutant animals. Total lysates were prepared from the indicated strains and were probed with anti-CHC, anti-α-adaptin, and anti-RME-1 antibodies in Western blots. Each lane is numbered. The signal intensity of each protein band was quantified and graphed (Bottom).