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. 2009 Jan 16;106(4):1139–1144. doi: 10.1073/pnas.0809541106

Fig. 5.

Fig. 5.

Normal synaptic transmission in neurons of chc-1(b1025ts) animals. chc-1(b1025ts) mini frequency and amplitude are statistically indistinguishable from wild type at 15°C and 30°C. (A) Event records from animals raised at 15°C or animals exposed to 30°C for 1 h. (B) Histograms of pooled event mini amplitude for each genotype and condition. At 30°C a slight increase in the distributions is seen for both wild type and chc-1(b1025ts) (5-pA bins). (C) Cumulative probability of pooled inter-event intervals showing mini intervals decreases with increased temperature for both wild type and chc-1(b1025ts) (5-ms bins). (D) Quantification of average mini amplitude and frequency from individual animals from each genotype and at each condition. (E) Aldicarb sensitivity assay. Forty L4 hermaphrodites of wild-type, chc-1(b1025ts) egl-30(tg26), and chc-1(b1025ts) egl-30(tg26) animals were incubated at 15°C and then placed on aldicarb plates (0.5 mM) at 20°C. The chc-1(b1025ts) mutant animals expressing GFP-CHC (dkEx6 [Prgef-1::GFP::chc-1]) under the control of a neuron-specific promoter also were examined. These animals were checked for their ability to move at the indicated time-points in response to prodding.