Fig. 3.

Cells with iNOS aggresome produce less NO. HEK293 cells were transfected with iNOS-GFP. (A) Twenty-four hours following transfection, cells were sorted according to GFP intensity. Cells not likely to be transfected and thus exhibiting lowest fluorescence intensity were not further used. Cells expressing moderate and the top 10% of fluorescence intensity were termed X₁ and X₂, respectively. (B) Sorted cells were cultured for 24 h and then subjected to live cell imaging in the presence of DAR. Representative fluorescence microscopy images of sorted cells show marked enrichment of aggresome-containing cells in the X₂ population. (C) Nitrite accumulation in tissue culture media of sorted cells. *, P < 0.05. (Scale bar, 10 μm.)