Fig. 6.

Altered cold-induced nociceptive behavior in Trpa1^−/− mice. (A) Latency to the first behavior reaction to cold upon placement on a cold plate at 0°C in mice of different sex and genotype. The numbers of mice tested on the cold plate were as follows: WT male (n = 13), WT female (n = 12), Trpa1^−/− male (n = 15), and Trpa1^−/− female (n = 13). (B) Average number of jumps during a 2-min period on a cold plate at 0°C. No jumps were observed when the plate was set to 30°C. (C) Cumulative probability plot showing the latency to the first jump off the cold plate in WT and Trpa1^−/− mice. (D) Tail-flick latency upon tail immersion in a water–methanol mixture at −10°C in WT (n = 32) and Trpa1^−/− (n = 34) male mice.