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ITC analysis of the interaction of heme with HasA-HasR. The binding isotherm derived from the signal is shown. The molar ratio heme/HasA-HasR is indicated. Thermodynamic parameters deduced from the fit are: Kd = 0.06 μM; ΔH = −36 kJ·mol−1; ΔG = −41 kJ·mol−1; TΔS = +5 kJ·mol−1.
