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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Stroke. 2005 Sep 1;36(10):2251–2257. doi: 10.1161/01.STR.0000181077.84981.11

Figure 2.

Figure 2

Effects of hydroxyfasudil (HFD) on eNOS activity, NO production, eNOS promoter activity, and eNOS mRNA stability. (a) HAEC was treated with the indicated concentrations of HFD for 96 hours. eNOS activity and nitrite release in culture media were measured (n=4). *P<0.05 vs basal condition. (b) Effects of hydroxyfasudil on eNOS promoter activity. BAEC were cotransfected with [-1.8 kb] eNOS promoter construct and pRL-CMV vector. Cells were harvested 48 hours after transfection and treated with the indicated conditions of HFD or exposed to laminar flow (12 dyne/cm2) (n=4). eNOS promoter activity was standardized to Renilla luciferase activity and expressed as fold induction. *P<0.05 vs basal condition. (c) Effects of HFD on eNOS mRNA stability. HAEC was treated with or without 10 μmol/L HFD in the presence of 50 μmol/L DRB (n=4).