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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Anal Biochem. 2008 Jul 8;381(2):258–266. doi: 10.1016/j.ab.2008.06.039

Table 2. Comparison of GST-Rab7 and GST-RhoA binding to BODIPY-GTP analogs.

Experiments carried out in 1 mM MgCl2 buffer

GTPase GST-Rab7 GST-RhoA
Nucleotide BODIPY FL BODIPY FL BODIPY FL BODIPY FL
GTP GTP-γ-NH GTP GTP-γ-NH
1Bmax (103 BODIPY/bead) 650 ± 10 60 ± 5 700 ± 20 710 ± 15
2Kd (nM) 20 ± 5 70 ± 20 80 ± 10 95 ± 10
1,2

Calculated as described in Table 1