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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Nat Genet. 2008 Mar 23;40(4):460–465. doi: 10.1038/ng.112

Figure 3.

Figure 3

[SWI+] can exist independently from [PSI+]. (a) Phenotypic analysis of a representative [psi-][SWI+] isolate derived from [PSI+][SWI+]-2 upon Hsp104 overproduction. After eliminating the HSP104 overexpression plasmid, [psi-][SWI+] cells treated with or without 5 mM guanidine hydrochloride were spotted onto indicated plates with a fivefold serial dilution. Pictures were taken after 5-d incubation at 30 °C. (b) Isogenic [psi-][SWI+] and [psi-][SWI-] cells containing pLS7 were treated with or without 5 mM guanidine hydrochloride and were grown in -tryptophan synthetic complete liquid media to mid-log phase before spotting to +sucrose -tryptophan media + 20 μg/ml X-gal (X-gal), +glucose -tryptophan media (-Trp) and YPD (YPD) plates. (c) HSP104 disruption eliminates [SWI+] in [psi-][SWI+] isolates. Shown are cell streaks of four isolates on YPD and raffinose plates before and after HSP104 gene disruption. Δ, HSP104 disruption. (d) The [SWI+] prion is tolerant to Hsp104 overproduction. Isogenic [psi-][SWI+] and [psi-][SWI-] cells containing p2HG (vector) or p2HGHSP104 (HSP104↑) were cultured and spotted to the indicated plates.

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