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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Nat Genet. 2008 Mar 23;40(4):460–465. doi: 10.1038/ng.112

Figure 5.

Figure 5

Swi1 but not Snf5 exists in distinct conformational states in [SWI+] and [swi-] cells. (a,b) Swi1-YFP was aggregated in [PSI+][SWI+] (a) and [psi-][SWI+] (b) but not in isogenic [swi-] cells or [SWI+] cells after guanidine hydrochloride treatment. Cells as indicated were treated with (+) or without (-) 5 mM guanidine hydrochloride. (c) c10B-H49 cytoductants of [SWI+] showed guanidine hydrochloride-curable fluorescent foci of Swi1-YFP. (d) Swi1-YFP was aggregated in the 74D-694 [psi-][SWI+] diploids but not in [psi-][swi-] diploids. (e) Swi1-YFP aggregates were mainly localized in cytoplasm. We stained 74D-694 [SWI+] cells in both [PSI+] and [psi-] backgrounds with Hoechst dye and analyzed them as described in Methods. (f) Endogenous Snf5-YFP is not aggregated in [SWI+] or [swi-] cells. Cells were treated with (+) or without (-) 5 mM guanidine hydrochloride and analyzed under a fluorescence microscope. All cells were transformed with p416TEFSWI1YFP and analyzed by fluorescence microscopy assays after growth in synthetic complete medium lacking uracil to mid-log phase.

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