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. Author manuscript; available in PMC: 2009 Feb 2.
Published in final edited form as: Eur J Neurosci. 2008 Apr;27(7):1585–1595. doi: 10.1111/j.1460-9568.2008.06119.x

Fig. 1.

Fig. 1

ADAM2 expression in the rostral migratory stream. (A) Nissl staining of a sagittal section from a postnatal day 5 (P5) mouse forebrain. The rostral migratory stream (RMS), which is characterized by high cellular density and intense Nissl staining, begins at the anterior portion of the subventricular zone (SVZa) and ends at the center of the olfactory bulb (OB). (B) ADAM2 immunoreactivity in the RMS. This is the adjacent section to that shown in (A, rectangular area), stained with 9D2.2, an anti-ADAM2 antibody. (C) ADAM2 mRNA expression in a frontal section (horizontal limb region; see legend for Fig. 2) from a P10 wild-type (WT) OB. (D) An adjacent section to that in (C) incubated with ADAM2 sense probe. (E) A frontal section of the OB from P10 ADAM2 knockout (KO) mouse incubated with ADAM2 antisense probe. (F) A section adjacent to (E) was incubated with the sense probe. (G and H) Frontal sections (vertical limb region; see Fig. 2 legend) of the OB from P10 WT mice were incubated with 9D2.2, anti-ADAM2 (green) and anti-polysialic acid (PSA) antibodies (magenta), respectively. (I and J) Frontal sections of the OB from P10 ADAM2 KO were incubated with anti-ADAM2 (green) and anti-PSA antibodies (magenta). GCL, granule cell layer. Scale bars: 500 μm. (K and L) Increased magnification of frontal sections from the WT RMS co-stained with anti-PSA and anti-ADAM2 (AB19030) antibodies. Scale bar: 10 μm. (M) Frontal section of the RMS from P21 WT mice co-stained with anti-glial fibrillary acidic protein (GFAP; green) and anti-ADAM2 antibodies (AB19030, magenta). (N) Frontal section of the RMS from P21 WT mice co-stained with anti-tenascin-C (green) and anti-ADAM2 antibodies (AB19030, magenta). (O) Frontal section of the RMS from P21 WT mice co-stained with anti-neurofilament monoclonal antibody (green) and anti-ADAM2 antibody (AB19030, magenta). Blood vessels containing IgG showed nonspecific staining for the secondary anti-mouse IgG antibody, but no specific staining for neurofilaments. Scale bar: 100 μm. (P) High magnification of (M). (Q) High magnification of (N). (R) High magnification of (O). Scale bar: 10 μm.