Fig. 5.

Disturbed tangential migration of ADAM2 knockout (KO) neuroblasts. Brain slices from a P10 mouse [wild-type (WT) or KO] were labeled with DiI and their migration recorded for 3–4 h. (A) Time-lapse sequence of WT neuroblasts migrating from the anterior part of the subventricular zone (SVZa; right bottom corner) toward the olfactory bulb (OB; left upper corner). The interval between each image is 30 min. See supplementary Video S1. (B) Higher magnification image of DiI-labeled WT neuroblasts migrating toward the OB. (C) Graphical representation of the migration of the neuroblasts in (A). Each point represents the position of the cell body at 5-min intervals. Arrows show the direction of neuroblast migration. (D) Time-lapse sequence of ADAM2 KO neuroblasts migrating from the SVZa (right) toward the OB (left). The interval between each image is 30 min. See supplementary Video S2. (E) Higher magnification image of DiI-labeled ADAM2 KO neuroblasts. (F) Graphical representation of the migration of the ADAM2 KO neuroblasts in (D). Each point represents the position of the cell body at 5-min intervals. Neuroblasts without a clear direction were marked with double-headed arrows. (G) Reduced migration rate of RMS neuroblasts from ADAM2 KO mice or in the presence of a function-blocking ADAM2 peptide. Each value is the mean ± SD. WT (N = 46, five slices from five mice), ADAM2 KO (N = 36, five slices from five mice), control peptide (N = 31, four slices from four mice), blocking peptide (N = 30, five slices from five mice). Statistically significant data (P < 0.05) are indicated by asterisks. Scale bars: 50 μm. (H) Distribution of migration speeds of the RMS neuroblasts from WT (N = 171, six slices from six mice) and KO (N = 202, six slices from six mice)mice.