. 2007 Jan-Mar;1(1):69–77. doi: 10.4161/pri.1.1.4060

Table 3.

Properties of hsp104 mutations

Domain	Mutation	[PSI⁺] Propagation^a	Thermotolerance^b	conservation (%)^c	Note^d	Ref. No.
	T8A	±	+	60	N	35
	L15Q	+	+	80	N	36
N-terminal	K20E	+	+	20	M	29
domain	A22V	+	+	80	M	29
	S89A	+	+	40	N	35
	L96Q	+	+	60	N	36
	D108A	±	+	40	N	35
	D184N	-	±	100	B	30
	D184Y	-	+	100	B	30
	A201V	-	+	0	M	29
	C209Y	-	±	100	N	27
	G212D	-	-	100	N	27
	G217V	-	-	100	T	38
NBD1	K218T	-	-	100	B	22,34
	A220T	-	±	100	N	27
	Y257A	-	+	100	T	7
	G259D	-	-	100	N	27
	E285A	-	-	100	N	31
	A298V	-	+	40	M	29
	T317A	-	+	100	B	32
	A341V	+	+	40	M	29
	G364N	+	+	60	M	29
D1-small	V365I	+	+	100	M	29
domain	P389L	-	±	100	N	27
	A392T	±	±	100	M	29
Middle	A430V	±	+	60	M	29
region	L462R	-	+	80	N	27
	T499I	-	±	0	M	29
	A503V	±	+	40	T	29,39
	A509D	-	-	20	T	29
	A531V	+	+	0	M	29
D1-small domain	A551T	+	+	60	M	29
	P557L	-	+	100	N	27
	V558I	+	+	60	M	29
	S597P	±	±	40	M	29
	G619V	-	-	100	T	38
	K620T	-	-	100	B	22,34
	E645K	-	-	100	T	7
	G661D	-	-	100	N	27
	Y662A	-	-	100	T	7
	P679L	-	±	100	M	29
NBD2	E687A	-	-	100	N	31
	D704N	-	+	100	N	27
	I722T	+	+	20	T	28
	T726I	-	+	100	M	29
	N728A	-	-	100	B	32
	S745P	+	+	0	M	28
	K747R	+	+	40	M	28
	R765A	-	-	100	N	31
	F772S	±	±	100	B	28
	K774E	±	±	0	B	28
	L775P	±	±	100	M	28
	L814S	+	-	80	B	28
C-terminal	R826M	-	±	100	B	33
domain	R830A	-	±	100	N	37
	L840Q	+	±	80	B	28
	L845I	+	+	80	M	28
	E878G	+	+	20	M	28

+, normal [PSI⁺] propagation; -, loss-of-[PSI⁺]; ±, weak [PSI⁺] propagation

The assessments were evaluated by three independent experiments. -, loss-of thermotolerance; ±, weak thermotolerance; +, normal thermotolerance.

The frequency of amino acid conservation at the indicated positions in S. cerevisiae Hsp104 relative to T. thermophilus ClpB, E. coli ClpA and ClpB, A. thaliana Hsp101 and S. cerevisiae Hsp78. Alignments refer to Lee et al.⁶

History about each mutations: N, newly engineered in this laboratory including those of very recent publication;²⁷ M, mutations reported in the literature as multiple amino acid substitutions and each mutation not clarified independently; T, mutations known to affect only thermotolerance; B, mutations known to affect both thermotolerance and [PSI⁺].