Figure 1.

Metabolic alterations in rice cultured cells induced by M. grisea elicitor. (A) Distribution of intracellular cationic metabolites in rice cultured cells treated with M. grisea elicitor. Metabolite signals were detected by CE/MS at m/z 100 to 600 in a positive ion mode. Relative differences in signal abundances between buffer- or elicitor-treated cells are expressed as the ratios on a logarithmic scale (log10-ratio). Positive and negative log10-ratio values indicate that the abundances were increased or decreased in elicitor-treated cells, respectively. The value close to zero indicates that the abundances were expressed at similar levels. (B) Effect of elicitor on titratable acidity in rice cells. White bars represent cells treated with phosphate buffer and black bars indicate cells treated with M. grisea elicitor. Titratable acidity was measured on homogenized of suspension cultured cells collected at 4 and 8 hrs after the treatments. Titration was performed by 0.01 N NaOH in the presence of phenolphthalein. Data are means ± SD from triplicated experiments.