Figure 1.

Alignment of deduced amino acid sequences of PLP homologs. The alignment was analyzed with the CLUSTALW program, and the amino acids were shaded black for 100% identity and gray for ≥50% identity by GENETYX software. (A) Full-length protein sequences of PLP homologs in Arabidopsis thaliana (AtPLPA, AtPLPB and AtPLPC), Oryza sativa (OsPLP), Solanum lycopersicum (SlPLP) and Physcomitrella patens (PpPLP) are compared. The PAS domain and the LOV domain are indicated with double and single lines, respectively, above the alignment. (B) PAS and LOV domains of PLP homologs are compared. Amino acids in phy3 LOV2 that interact with flavin are indicated by solid triangles⁹. The position corresponding to the cysteine residue involved in formation of the covalent adduct with flavin in phy3 LOV2 is marked with an asterisk.