Figure 1.

Effect of lipid metabolism inhibitor (cerulenin) on the polarity establishment in A. nidulans. Wild-type conidia grow isotropically until the first mitotic division (not shown) and then send a polarized germ-tube to establish the filamentous hyphae characteristic of A. nidulans. In the presence of 20 µg/ml of cerulenin, conidia exhibit an extended isotropic growth because of a delay in polarity establishment. In 50 µg ml of cerulenin, conidia exhibit isotropic growth but do not extend a germ tube. Scale = 10 µm. Objective numerical aperture = 1.4 with oil.