Figure 2.

Northern-blot analysis of CYP86A33 in potato organs and tuber tissues. Northern-blot membranes and ethidium bromide-stained gels are shown in the upper and lower panels of each image, respectively. A, Transcript profile in potato: stem (S), leaf (L), root (R), tuber parenchyma (T-PAR), and tuber periderm (T-PER). Note that the CYP86A33 transcript accumulates only in suberized tissues: roots and tuber periderm. B, Transcript accumulation in tuber periderms of three independent CYP86A33-RNAi transgenic potato plants in comparison to wild type. Periderms from these three lines were selected for chemical and water permeability analyses.