Figure 4.

A, Northern-blot analysis of ad1 and ad-su6. Cells were adapted to L-CO₂ or VL-CO₂ conditions for 4 and 14 h, respectively, and 10 μg of total RNA was used. B, Immunoblot analysis of whole cell fractions. Cells were either H-CO₂ grown or induced 24 h under L-CO₂ (L24) or VL-CO₂ (VL24) conditions. One hundred micrograms of total protein were analyzed with CAH3 antibody. su6-C1 and su6-C3 are two CAH3 genomic DNA-complemented ad-su6 transformants as described in Figure 2.