Figure 8.

Analysis of genes encoding transcription factors that displayed germination-specific expression. A, Analysis of the expression profiles of 34 transcription factors that displayed between 70% and 100% of their maximum expression at 1 and 3 HAI in the germination time course, compared with publicly available array data for a variety of rice tissues and treatments. Boxed in yellow are transcription factors that appeared only to be induced during germination (i.e. in this study). Boxed in blue are transcription factors only expressed during germination, coleoptiles, or suspension cells, and boxed in green are transcription factors only expressed in suspension cells and in this study. Asterisks indicate genes for which the Arabidopsis homologs have germination-specific expression (see B). B, inParanoid (Remm et al., 2001) and GreenPhylDB (Conte et al., 2008) were used to identify Arabidopsis homologs for the rice transcription factors defined as germination specific (see A). Their expression profiles in seed germination were investigated using the Arabidopsis eFP browser (Winter et al., 2007). Two Arabidopsis transcription factors that showed transient and germination-specific expression were identified, including a WUSCHEL-related homeobox transcription factor (At3g03660) homologous to rice homeobox transcription factors encoded at the loci Os08g14400, Os03g20910, and Os07g48560 and a zinc finger homeodomain transcription factor (At5g15120) homologous to the rice zinc finger homeodomain transcription factor Os09g29130.